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Measurement of extracellular acidification rate (ECAR) NSCLC cells (2 10 4 cells/well) were seeded in Seahorse XF24-well microplate (Agilent)

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But one appeals court, the ultra-conservative Fifth Circuit Court of Appeals, rejected virtually every aspect of the FDA's decision-making process, eviscerating the agency's conduct as a "surprise switcheroo" in which companies were told that the FDA required certain studies, including marketing studies, but then changed its mind