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Ethics approval This prospective singlearm, selfcontrolled trial was conducted at the IVF Center of Nanjing Drum Tower Hospital, affiliated with the Medical School of Nanjing University, from October 2023 to October 2024

Compound Information: Sequence: Ala-His-Lys:Cu (tripeptide-copper complex) Classification: Synthetic copper peptide Product Specifications: Form: Lyophilized (freeze-dried) powder Purity: 98% (HPLC verified) Container: Type I Borosilicate Glass Vial Storage: -20C recommended, 2-8C for short-term Reconstitution: Bacteriostatic water or sterile water Manufacturer: NovumLab Quality Control: Each batch independently tested Intended Use: In-vitro laboratory research only This product is not a drug, food, or cosmetic and should be handled by qualified professionals in laboratory conditions

Commonly reported was a biofilm with a clumped or flake-like appearance, with more extensive injuries showing broken and loss of branches and bare skeletons (Etnoyer et al., 2016

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