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Yeast cells (5 10 5 /ml at the start) were routinely cultured in YPD medium (1% yeast extract, 2% soybean peptone and 2% glucose) (Sigma-Aldrich) for 18 h at 30C with shaking (170 rpm) using an orbital rotary shaker MaxQ 6000 (ThermoFisher Scientific, Waltham, MA, United States)

MTHFR gene C677T polymorphism increases the genetic predisposition to hyperhomocysteinemia

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Anti-Aging Effects: Its antioxidant properties reduce oxidative stress, minimizing fine lines and promoting skin firmness

The difference is purely in how many total doses you extract before needing a new vial