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After 30 minutes of incubation at 37 C in the dark, the percentage of PI positive cells was determined by using a flow cytometer (BD FACSCanto II, BD Biosciences), with 488 nm excitation laser and 585/42 nm band pass filter

The advent of fear conditioning as an animal model of post-traumatic stress disorder: learning from the past to shape the future of PTSD research

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Before bed: The caffeine is small but can affect light sleepers