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The gel was crushed and the DNA was eluted twice from the crushed gel with 200 L of diffusion buffer (10 mM Tris, 25 mM sodium chloride, 1 mM EDTA, pH 8), at 70 C for 1 hour followed by 30 minutes, with mild shaking (1500 rpm)

Growth hormone plays a fundamental role in preserving lean tissue during caloric deficit

Mice with hepcidin-resistant ferroportin accumulate iron in the retina

Okamoto A, Iwamoto Y, Maru Y

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