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Am J Physiol 258 :G185G190

(2023) 189:G4364

Bacteriostatic water contains a preservative that inhibits bacterial growth, extending the vials life to about a month

Key Safety Considerations Despite its biological activity, IGF-1 LR3 raises several concerns: Blood glucose disruption: hypoglycemia is a known risk Fluid retention and musculoskeletal discomfort: commonly reported Metabolic effects: potential insulin resistance with extended exposure Oncogenic signaling: persistently elevated IGF-1 levels have been linked to certain cancers Regulatory Reality Not FDA-approved for therapeutic or lifestyle use Banned in competitive sports by anti-doping agencies Limited clinical evidence: lacks robust, long-term human safety trials A Cautious Outlook While IGF-1 LR3 provides valuable insights into growth factor biology, its translation beyond controlled research environments remains unresolved

The co-trimoxazole was dissolved in BG11 medium to give a concentration of 1 10 3 ppm as stock solution