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Although methotrexate is one of the first examples of intelligent drug design, multiple mechanisms potentially contribute to the anti-inflammatory actions of methotrexate, including the inhibition of purine and pyrimidine synthesis, transmethylation reactions, translocation of nuclear factor-B (NF-B) to the nucleus, signalling via the Janus kinase (JAK)signal transducer and activator of transcription (STAT) pathway and nitric oxide production, as well as the promotion of adenosine release and expression of certain long non-coding RNAs

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This was also observed in this study in the magnesium treated group with values obtained being comparable to those of the insulin treated animals (Figure 1)

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Targeting oxidative stress and inflammatory response for blood-brain barrier protection in intracerebral hemorrhage