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Mutat Research/Fundamental Mol Mech Mutagenesis

Immunogenicity of therapeutic proteins: clinical implications and future prospects

pClc, corresponding to the C 334 V mutant of pNp, was generated via QuikChange site-directed mutagenesis (Phusion polymerase, Thermo Fisher Scientific [TFS]) using pS6-proNEP as a template and specific forward (5GCG TGA CCT GCA GGT CGT TGA TAC CTA TGG3) and reverse (5CCA TAG GTA TCA ACG ACC TGC AGG TCA CGC3) primers to yield the pS6-pro-celiacase plasmid

We thank Len Patt of the ProCyte Corporation (Redmond, WA) for making a complimentary sample of the copper tripeptide available for our investigation

With BPC-157, the benefits are unproven and the risks unknown a scenario that challenges the ethical boundaries of clinical practice