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1 Introduction Metabolic reprogramming stands as one of the hallmarks of malignant tumors, enabling tumor cells to remodel core metabolic pathways to meet the demands of rapid proliferation, invasion, metastasis, and adaptation to microenvironmental stress ( de novo synthesis pathway (SSP), which becomes the primary intracellular source of serine in tumor cells ( In recent years, with the advancement of pan-cancer studies, aberrant activation of the SSP has been successively identified across various malignant tumors, and its biological functions and regulatory mechanisms exhibit remarkable cancer-type heterogeneity ( Against this background, this review begins with the metabolic mechanism of the SSP, and elaborates in detail on its core functions in pan-cancer, multi-level regulatory networks, as well as current therapeutic strategies and challenges targeting the SSP, aiming to provide a reference for subsequent tumor metabolism research and clinical translation

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In addition to the above, DSIP has demonstrated some potential as a therapy for other health concerns, at least in animal studies

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The pellets were resuspended by addition of 90 l of buffer containing urea (8 M), TCEP (5 mM) and Triethylammonium bicarbonate (TEAB, 50 mM, pH 8.5) with sonication