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doi: 10.1038/s41573-023-00822-2 94 LuoXBaoXWengXBaiXFengYHuangJet al

Overproduction of the RNS above the ability of the cell to neutralize them is known as nitrosative stress and may lead to nitrosylation reactions that can alter the structure of proteins and so inhibit their normal function [7]

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Next, the apo-enzyme was eluted using GST elution buffer (50 mM Tris, 10 mM methylglutathione, pH 8) before relevant fractions were combined according to their A 280nm

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