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To analyze glutamate in the supernatant, the culture supernatant was diluted 10-fold with methanol and transferred to a UFLC Nexera system (Shimadzu) connected to a Triple TOF 5600 system (SCIEX, Tokyo, Japan)

However, reported use of methamphetamine without use of any additional drugs increased by 43% during the same time period

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DDAH is upregulated in IPF, leading to decreased ADMA levels and increased NOS2 activity, thereby enhancing NO production and contributing to fibrotic progression [81]