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Active tissue-specific DNA demethylation conferred by somatic cell nuclei in stable heterokaryons

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MIA PaCa-2 cell was cultured in DMEM (Gibco, 11995500), which contains 10% heat-inactivated fetal bovine serum (FBS

For the IFN-I bioassay, 5 x 10 4 3T3 cells containing an interferon-sensitive response element (ISRE) fused to luciferase 51 were plated per well into 96-well white-bottom plates (Greneir 655083) in DMEM containing 10% FBS, 100 U/ml penicillin and 100 g/ml streptomycin