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GSH serve as co-factor for glutathione peroxidase-4 (GPx4) enzyme which catalyzes the conversion of lipid peroxides to lipid hydroxides thereby preventing iron-mediated cell death

Notably, the NPC1 -knockout cells yielded the majority of both sensitizing and desensitizing hits (65% and 77%, respectively), consistent with the damage-prone state of this genetic background

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Of note, Gpx4 mRNA levels were downregulated in tissues from Apc +/Min /Stard7 IEC , which is in contrast to elevated Gpx4 protein levels found in these tissues, which possibly reflects the involvement of post-translational modifications of Gpx4 occurring upon Stard7 deficiency (Fig