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Following 6 min of Ca 2+ -free NT perfusion, the solution was exchanged for the Ca 2+ -free NT solution supplemented with 0.82 mg/mL collagenase (Type I), 0.5 mg/mL protease (type XIV 15% Ca 2+ ), and 1.67 mg/mL BSA prepared from factor V albumin (all purchased from Merck, Gillingham, United Kingdom) for 48 min until rod-shaped cardiomyocytes were visible in the perfusate

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2 Succinate supplementation or TMLHE inhibition decrease resistance to DNA damaging agents

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10.1074/jbc.273.18.10815 11 ChenH.IglesiasM