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Overall, the elongated ASXL1 EBM -binding groove, delineated by I622, L630, V633, V634, I652, I654 and F656 of BRD4 ET , is mainly hydrophobic but is surrounded by the negatively charged walls created by the residues of the 1 strand and the 2-3 loop of BRD4 ET , such as D650, E651, E653, D655 and E657 (Fig

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