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The sections were then incubated with Alexa fluor 555-labeled secondary antibody (1:1000 in 1x PBS pH 7.4) (Invitrogen, Thermo Fisher Scientific) for 1 hour at room temperature in a dark, humidified chamber, followed by washing in PBS

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On Jupiter, its mushballs all the way down Slushy hailstones of ammonia and water were part of a bizarre theory to explain the planet's poorly mixed atmosphere