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For immunofluorescence, sections were washed with PBS at room temperature twice for 10 min, followed by incubation in blocking buffer (5% fish gelatine, 0.5% Triton in PBS) at room temperature for 1 h before incubation with the primary antibodies (see Supplementary Table 4 for primary antibodies used in this study) diluted in the blocking buffer at 4 C overnight

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Phase Three During the third phase of the HCG diet, which lasts three to four weeks, you will maintain your new weight

144 PEGDA hydrogel scaffolds fabricated using SLA exhibit comparable thermal and chemical properties to UV-cured hydrogels