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After separation by gel (10% SDS-PAGE) electrophoresis, the proteins were electrotransferred to PVDF membranes, blocked with 5% skim milk for 1 h, and incubated with antibodies against -tubulin (1:1000, Cell Signaling), MRP2 (1:1000, Cell Signaling), HIF-1 (1:1000, Cell Signaling), MMP9 (1:1000, Cell Signaling) and RKIP (1:1000, Cell Signaling) overnight at 4

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Consistent results were observed in the primary cell culture from neuroendocrine ovarian cancer (Supplementary Fig