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Shikonin triggers GSDME-mediated pyroptosis in tumours by regulating autophagy via the ROSMAPK14/p38 axis

This cascade results in the cleavage of Gasdermin D, leading to the formation of membrane pores that may contribute to cell lysis and the release of the proinflammatory cytokines interleukin (IL)-1 and IL-18

Six hours after transfection the medium (DMEM, 10% FBS, 2mM L-glutamine without antibiotics) was replaced, and cells incubated for 18 hours before the medium was harvested for viral infection of recipient cells

proposed that LPS-induced inflammatory response and oxidative stress are predictive of progression to early compensated cirrhosis and end-stage liver failure in patients with chronic HBV or HCV infection (Sandler et al., 2011)

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