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,Biodiesel is most often defined as ,the mono alkyl esters of long chain fatty acids derived from renewable vegetable oils or animal fats for use in compression ignition (diesel) engines [37]. Commercially available biodiesel is a mixture of fatty acid methyl or ethyl esters, produced by transesterification of triglycerides, and should not be a significant source of glycerol when properly refined

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Passage number of cell lines is between 2-3 times a week for a maximum of 4 weeks