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2.2 Cell culture and analysis of cell proliferation Lymphoblasts were grown in RPMI 1640 medium from Sigma-Aldrich (Saint Louis, MO, USA, R6504) supplemented with 2 g/L sodium bicarbonate, 15% (v/v) fetal bovine serum and 0.5% (v/v) of penicillin-streptomycin plus amphotericin B in T25 or T75 flasks, in an upright, at 37C in a humidified atmosphere of 5% CO 2

These two cited studies further note that successive ligations of the two strand breaks are best achieved if the DNA helix moves (rotates or changes twist, and/or translocates), relative to the rest of the complex

N-Acetyl Cysteine (NAC) is a potent antioxidant that helps eliminate free radicals, and repair cellular damage

10.1016/j.rbmo.2011.08.010 44

10.1016/j.freeradbiomed.2016.02.021 77 PrasetiaR.PurwanaS