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Upon confluence of the seeded HCE-2 cells in the culture flask, the cells were trypsinized with Trypsin-EDTA, then trypsinization was stopped with a complete growth medium and cells were harvested, counted and seeded into the six prepared wells

I Physicochemical compatibility between the chitosan used as a mucoadhesive agent and the methylcobalamine is carried out by making physical mixture with kBr pellet and allow them to react for 24 hr in dark condition them in this mixture analysed and infrared radiation from 400-4000 cm

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