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RNA from the barcoded cells was subsequently reverse-transcribed, and sequencing libraries were constructed using a Chromium Single Cell 3 (v3) reagent kit (10x Genomics, PN-1000269) following the manufacturers instructions

Contact with NNA led to DNA damage, including double-strand breaks, the most deleterious genotoxic outcome. Tested in mice, rats, hamsters and mink, researchers noted that NNK caused cancer, including of the liver, lung and pancreas, suggesting that its presence could have toxic effects on humans

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