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However, some patients still feel that the deeper structures of the face lack firm support almost as if the skin and underlying tissue are not fully connected

Cell pellets were lysed for 10 min in 600 L of lysis buffer [20 mM Tris-HCl pH 8.0, 100 mM NaCl, 6 mM MgCl 2 , 1 mM EDTA, 1.5% (v/v) Triton X-100 (MilliporeSigma, T9284), 60 mM octyl B-D-glucopyranoside (MilliporeSigma, O8001, 1 mM phenylmethanesulfonyl fluoride solution (MilliporeSigma, 93482), and 1X Halt Protease Inhibitor] and 1 L Pierce Universal Nuclease (Thermo Fisher, 88702) per 5 10 7 cells

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doi: 10.7759/cureus.86527