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To further investigate the transcriptional changes in functionally coordinated genes, Gene Set Enrichment Analysis (GSEA) 33 was performed using the Kyoto Encyclopedia of Genes and Genomes (KEGG) functional gene sets available in MSigDB ( The top 10 downregulated and 10 upregulated KEGG gene sets in GLA-mutant Fabry kidney organoids compared with WT kidney organoids are shown in Supplementary Table 2

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