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J Alzheimers Dis 30(4):943961 Escribano L, Simn AM, Gimeno E, Cuadrado-Tejedor M, Lopez de Maturana R, Garca-Osta A, Ricobaraza A, Prez-Mediavilla A, Del Ro J, Frechilla D (2010) Rosiglitazone rescues memory impairment in Alzheimers transgenic mice: mechanisms involving a reduced amyloid and tau pathology

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Briefly, 10 6 BEAS-2B cells or HBEC were recovered using EDTA-free trypsin, counted and incubated in serum-free DMEM with 0.2 M Calcein-AM at 37 C for 7 min