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Using a murine model of heart failure, Xu et al 157 demonstrated that the soluble epoxide hydrolase inhibitor 1-adamantan-3-(5-(2-(2-ethylethoxy)ethoxy)pentyl)urea could effectively enhance the biological activities of EETs and thus improve pressure overload- or Ang II (angiotensin II)-induced myocardial hypertrophy and ventricular arrhythmias

J Neurochem 96 : 12671276

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The cells were fixed for 1 h in 0.1 M Na-cacodylate buffer pH 7.2 containing EM-grade 2.5% glutaraldehyde and 2% formaldehyde, followed by washing and incubation in 1% osmium tetroxide and 1.5% ferricyanide for 1 h in the same buffer