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Subsequently, cells were centrifuged at 4 C, 4,000 g for 15 min, then resuspended in cold buffer A (20 mM HEPES-Na + , pH 8.0, 500 mM NaCl, 1 mM TCEP) and sonicated three times for 15 min each

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The mitochondria used in the study were isolated from the heart of inducible Coq7 knockout (KO) mice (123)

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2005) and in the plasma (Monserrat-Mesquida et al