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People who dont eat meat, which is a natural source of B12, are at risk of B12 deficiency, including vegans and vegetarians

Primers used in 5 to 3 direction: sVEGFR1 forward:AGAGGTGAGCACTGCAACAA, sVEGFR1 reverse: TCTCCTCCGAGCCTGAAAGT, UBC forward: AGTAGTCCCTTCTCGGCGAT and UBC reverse: GCATTGTCAAGTGACGATCACAG