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doi: 10.1016/j.cocis.2020.05.002

You can also watch episodes on YouTube at My profound breath-work session w/ Damian "Wim" Glynn [PART TWO] Part 1 was the theory, now we're putting it into practice

The F2 progeny was subsequently screened for the desired edit by PCR amplification using the adh-5 forward primer (5-CGATCCAAGTGGCTCCACCGAA-3) and the adh-5 reverse primer (5-TTCCACATCCCAAAAGCGAAACC-3)