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Materials and methods Animal experiments C57BL/6J aged (20 months) male mice and mast cell deficient c-Kit wsh /cKit wsh mice (12 months) were housed in a specific pathogen-free facility (light cycle 12/12 h light/dark)

GAPDH was used as an internal reference for normalization

There is insufficient safety data for either compound during pregnancy, and animal studies with GLP-1 agonists showed adverse fetal effects

142 Stabilization of HIF1A by hypoxia or cobalt chloride promotes fatty acid uptake and lipid storage by transcriptional upregulation of fatty acid-binding protein 3 (FABP3) and fatty acid-binding protein 7 (FABP7), and ultimately inhibits ferroptosis in human fibrosarcoma or lung cancer cells through increasing lipid droplet formation

In particular, there was no significant rise in Cho and decrease in NAA peak, no reversal of the tNAA/tCho ratio, and no notable lactate or lipid peak