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Given that both diet composition and adiposity can each substantially impact the gut microflora in obese mice 24 , we conducted a secondary endpoint study using animals given the same series of diets to test the hypothesis that NNMTi treatment combined with LD, but not LD alone, shifts the cecal microbiome profile of obese animals to be similar to age-matched LD controls that were never given a high-fat diet

Its also important to monitor B12 levels periodically through blood tests, as this helps adjust the treatment plan to ensure the best possible outcomes while avoiding over-supplementation

Experts do not generally recommend discontinuing the use of metformin for people with new cases of vitamin B12 deficiency

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