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GDH1 regulates redox balance by controlling intracellular levels of -KG and glutaminolysis (42)

Taken together, the stabilization of HIF-1 promotes pathogen clearance in mycobacterial infection by the remodeling of metabolic pathways in macrophages, and the effect of HIF-1 can be beneficial or detrimental to the host depending on the infection stages or immune cell activation states ( Table 3 )

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The cell pellets were lysed by sonication in ice-cold PBS containing 0.1% TritonX-100, centrifuged at 20,000 g for 30 min to remove cell debris, and protein concentrations were determined by BCA protein assay