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For cell activity, the CCK-8 solution and corresponding medium were added to the wells, incubated at 28C for 2 hours, and absorbance was measured at 450 nm

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Yamamoto Y, Konishi H, Ichikawa D, Arita T, Shoda K, Komatsu S et al

Ice therapy in the first 48 hours and heat therapy afterward can speed recovery

Fuller M, Whiley H, Kper I (2020) Antibiotic delivery using gold nanoparticles