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doi: 19 10.1111/j.1538-7836.2010.03941.x

The primers 5-ACC CCT CGT TTT ACA TCT-3 and 5-TTT GGA GTC CTT CCT TTC-3 were used to amplify the SIRT1 distal promoter sequence (A1, nucleotides 974 to 843), and the primers 5-ACC CAA CAA ACC CAT TCT-3 and 5-CCT CCT GGG AAG ACC TTT-3 were used to amplify the SIRT1 proximal promoter sequence (A2, nucleotides 781 to 647)