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Forebrains free of meninges were dissociated with Hanks' Balanced Salt Solution containing 0.01% trypsin and 10 g/ml DNase, and triturated with DMEM containing 10% heat-inactivated fetal bovine serum and 100 U/ml penicillin and 100 g/ml streptomycin

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MRI provides the soft-tissue detail the X-ray cannot: cartilage thickness across the full joint surface, the condition of the subchondral bone beneath it, meniscal integrity, and ligamentous stability

We also observed increased lipid ROS levels in CD8 + T cells after A2ARi treatment with addition of exogenous GSH (Supplemental Figure 5, C and D), consistent with the imbalance of the ratio of GSH to GSSG during oxidative stress (Figure 2G)

Volcano plots were made using the FC = 1 and p value 1 were represented as dot plots generated using ggplot2, dplyr, and tidyr