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In brief, proteins in placental lysates (preparation see "Iron content analysis by colorimetric ferrozine-based assay" section) were isolated by TCA precipitation as described above for the TBARS assay

Furthermore, SIH mitigated the glutathione depletion induced by DEM in Day four animals (Figure 1F), demonstrating that cytosolic iron synergizes the depletion of glutathione initiated by DEM

Consequently, next-generation sensor development with enhanced sensitivity, specificity, spatial resolution, multiplexing capacity, and biocompatibility is most paramount for groundbreaking developments in redox biology and in translating knowledge into effective diagnostics and therapeutics

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For comparison, we also analyzed the effects on transcription when cells were treated with H 2 O 2 or with benzoquinone, which are chemically very different from HOCl