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were detected and counted by fluorescent microscopy (400 magnification) after incubation with a Rhodamine Red-X goat anti-mouse IgG (H+L) (Invitrogen)

In rodents, enteral administration of acetate and butyrate has been shown to induce the expression of beta-defensin and histone inhibitor-related antimicrobial peptides in intestinal epithelial cells in a GPR43-mediated manner as a means of protecting the intestinal barrier and organizing the inflammatory response induced by, among others, LPS (173)