However, gut microbial formation and catabolism of BB during oral l-carnitine metabolism in omnivores versus vegans/vegetarians, the human commensals that can utilize BB as a nutrient, and the relationship of this metabolite to the metaorganismal TMAO pathway and CVD had not yet been explored
Evans AM, Fornasini G

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To determine hydration levels, creatinine concentration was quantified using the Creatinine (urinary) Colorimetric Assay Kit (Cayman Chemical Co., Ann Arbor, MI, USA) as described by the manufacturer
Gene expression of IL-8 and IL-6 was evaluated by qRT-PCR conducted at QuantStudio 3 Real-Time PCR System (Thermo Fisher Scientific, Waltham, MA, United States) using specific FAM-labeled probe and primers (prevalidated TaqMan Gene expression assay for IL-8, Hs00174103_m1