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the freeze-drying process comprises the following steps, preparing a liquid and filling at the temperature of 20-30 , putting a sample into a freeze dryer, quickly reducing the temperature of a plate layer to-40 , preserving heat for 1h, when the temperature of a clapboard is-10 +/-2 , preserving heat for 1h, quickly reducing the temperature of the plate layer to-40 within 1h, preserving heat for 2h, transferring to a cold trap at-50 , vacuumizing to below 10Pa, firstly preserving the temperature of the plate layer to-5 for 4h, then heating the plate layer to 10 within 3h, when a product is at 0 , starting to increase the temperature of the plate layer to 40 , and when the temperature of the product is increased to 30 , preserving heat for 4 h

A mutation in the HFE gene is associated with altered brain iron profiles and increased oxidative stress in mice

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All other hits had Z-scores below 10, including the FH-binding protein CspA (PDB: 1w33, 4bl4) 31,32

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