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Determination of resistant bacteria for ozone treatment After identifying the typical Escherichia coli and Pseudomonas aeruginosa bacteria from specific culture media, to confirm the identified colonies, the isolated bacteria were accurately identified by PCR and based on the BLAST results, five types of Pseudomonas aeruginosa and two types of Escherichia coli were confirmed, and all Pseudomonas aeruginosa and Escherichia coli were exposed to different concentrations of antibiotics (8128 g/ml) and examined for the presence of antibiotic resistance genes

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