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Gene expression was normalized to the mRNA expression level of -actin as an endogenous control, and fold changes were calculated between treatment and negative-treatment control samples

Prepare three density gradient solutions of 1.03, 1.04 and 1.06 g/ml (equivalent to 5.4%, 6.4% and 10.3%, w/v iodixanol) by diluting Optiprep with Solution B (see Note 2)

This review provides time bound framework, which looks at stepwise management of acute severe ulcerative colitis and explores the recent concepts of choice between biologics and cyclosporin colon rescue therapies in case of steroid refractory disease

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