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Healthy controls were required to be free from any current or past history of significant psychiatric disorder on DSM-5 and not taking any psychotropic medications

J.FieldingK.CharalambousS.DayJ

It has been theorized that the chemicals in coffee enemas cause free radicals to be absorbed by the sulfhydryl (-SH) groups of glutathione in the bile, which thereafter become bile salts that are flushed out of the gall bladder [20]