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Genomic DNA from these cells was isolated with the QIAamp DNA Blood Maxi Kit (Qiagen #51192) followed by PCR amplification using Illumina adapters to identify the gRNA representation in each cell

Jianfang W, Raza SHA, Pant SD, Juan Z, Prakash A, Abdelnour SA et al (2025) Exploring epigenetic and genetic modulation in animal responses to thermal stress