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doi: 10.1042/BJ20110841 7 ShibuyaNKoikeSTanakaMIshigami-YuasaMKimuraYOgasawaraYet al

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After removing half of the culture medium, neurons at 6070% confluency were transfected by adding 100 l transfection mix per 1.76 cm 2 well dropwise onto the cells