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The purified OT-1 CD8 + T cells were then maintained in DMEM-based culture medium supplemented with IL-2 (10 ng ml 1 , 212-12, PeproTech), T-Select H-2K OVA Tetramer (Medical & Biological Laboratories) and -mercaptoethanol (100 mM, M3148, Sigma-Aldrich) and cultured for 35 days

Glutathione is involved in cellular metabolism and oxidises quickly

He was most recently Senior Managing Director and Head of Europe at Stonepeak and previously spent 22 years at Macquarie, where he served as Global Co-Head of Macquarie Capital and Chairman of the Green Investment Group

doi: 10.1111/j.1550-7408.1988.tb04115.x 25 TanJSackBKOyenDZenklusenIPiccoliLBarbieriSet al

Pan Afr Med J 2021;38:237