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doi:10.1016/j.exger.2014.12.016 Gebara KS, Gasparotto Junior A, Palozi RAC, et al

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The phosphorylation levels of MPK3 and MPK6 were quantified with the blot analysis plug-in in ImageJ ( Treatment with the calcium ionophore A23187 Twelve-day-old Col-0 seedlings were treated with 50 M calcium ionophore A23187 (Sigma-Aldrich Co., MO, USA) for 15, 30, and 60 min

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Ethics declarations Ethics approval and consent to participate All animal studies were approved by the Institutional Animal Care and Use Committee (IACUC) of Huazhong University of Science and Technology (IACUC approval number: 2585)