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The following day, Ni-NTA resin equilibrated in buffer A was added over the cleaved laccase samples and incubated at 25 C and 1,000 rpm in a thermomixer comfort shaker (Eppendorf) for 4 h

The MedDiet has been reported to provide approximately 43 % energy from carbohydrate, 37 % energy from fat and 15 % from protein (Reference Davis, Bryan and Hodgson34)

doi: 10.1073/pnas.87.9.3547

In contrast, metabolites such as phosphatidic acid, phosphatidylglycerol (16:0/0:0), PC (16:0/3:1(2E)), and phosphatidylinositol (16:0/16:0) are significantly upregulated, suggesting a high saturation of glycerophospholipids

[DOI] [PubMed] [Google Scholar] 791.Lopez CJ, Qayyum I, Mishra OP, Delivoria-Papadopoulos M