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200 l of culture broth was combined with 4 volumes of pure ethanol, then centrifuged at 7500 rpm for 9 min

the building block polynucleotide can further comprise a nucleic acid sequence capable of introducing one or more nucleotide residues into the template polynucleotide, or, deleting one or more residue from the template polynucleotide

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Specifically, in addition to the routine capping of the -amino group with Fmoc or Boc, for the chemically more variable side-chain -amino group, chemists must flexibly select protecting groups according to the deprotection requirements of a given synthetic route, including acid-labile Boc, base-labile Fmoc, hydrogenation- or strong-acid-labile Cbz, palladium-removable Alloc, or hydrazine-sensitive IvDde