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Of these, 100 genes overlapped between treatments (Figure 1A)

Apoptosis assay (flow cytometry) The apoptosis rate of Raji cells was determined by flow cytometry using a dead cell apoptosis kit with Annexin V Alexa Fluor 488 and propidium iodide (Invitrogen)

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Mice infected with the D39 gpoA mutant strain exhibited a significant reduction in bacterial loads across multiple tissues, including the lung, brain, liver, and spleen, relative to those infected with the wild-type strain